Cylindrus obtusus

Communication: Luise Kruckenhauser (genetics), Laura Zopp (anatomy, morphology, histology)

Cylindrus obtusus

The hermaphroditic land snail Cylindrus obtusus (Draparnaud, 1805) is an endemic of the Austrian Alps. Restricted to high elevations (1600 to 2500 m asl; Bisenberger et al. 1999, Duda et al. 2010) it has a rather patchy distribution extending from Schneeberg to Großglockner. Previous qualitative analyses of anatomical traits of the distal female genital tract revealed two geographically distinct groups. Western populations show pairwise mucus glands of equal size whereas in eastern populations these glands are conspicuously shortened and (sometimes) asymmetrically to different degrees (Schileyko et al. 1997). To find out whether these anatomical differences reflect a genetic differentiation, which might be an indication for distinct glacial refugia, we investigate a partial sequence of the mitochondrial COI gene and 9 microsatellite loci (Arthofer et al. 2010) from samples covering the whole distribution range of the species. The COI sequences indicate a geographic differentiation between eastern, central and western populations. However, genetic distances are small (max. 1.7 %). The microsatellite analysis reveals a high differentiation between the populations implying restriction of gene flow. Remarkably, nearly all individuals from the eastern populations are homozygous at all microsatellite loci (although different alleles were found within populations). The most plausible explanation for this finding is an altered mode of reproduction. Further investigations shall elucidate whether this lack of heterozygotes is caused by selfing and if so, why it occurs at such a high frequency. In a morphological study two different approaches are employed: Quantitative measurements of the mucus glands and the stylophore are taken from more than 70 individuals collected from 16 populations. Preliminary results confirm quantitatively the east-west division previously described. A comparative histological investigation of the mucus glands will be performed to assess the functionality of these structures in both groups.

In an additional analysis we try to elucidate the phylogenetic relationships of C. obtusus within the Helicidae on the basis of various molecular markers.

First results were presented at several scientific meetings.

Luise Kruckenhauser

Luise Kruckenhauser

Natural History Museum of Vienna, Central Research Laboratories

Email: Luise.Kruckenhauser@nhm-wien.ac.at

Read more about Luise Kruckenhauser

Laura Zopp

Laura Zopp

Natural History Museum of Vienna, Central Research Laboratories

Email: laura.zopp@nhm-wien.ac.at

Read more about Laura Zopp

Publications on Cylindrus obtusus

Thesis

Kleewein, D., Baumgartner, G., & Sattmann, H. (2000). Erste Beobachtungen zur Reproduktionsbiologie von Cylindrus obtusus (DRAPARNAUD, 1805)(Pulmonata, Helicidae). In H. Sattmann, D. Kleewein, & H. Kothbauer (Eds.), Arianta III - Berichte der Arbeitsgruppe Alpine Landschnecken (p. 49). Wien (Austria): Naturhistorisches Museum. Cite
Zopp, L. (2012). Geographische Variation in der Schalenmorphologie, Anatomie und Histologie von Cylindrus obtusus (Diploma Thesis). University of Vienna, Vienna, Austria. Cite

Journal Articles

Arthofer, W., Cadahía, L., & Kruckenhauser, L. (2010). Ten new microsatellite loci for analysis of genetic diversity in isolated populations of the Alpine land snail Cylindrus obtusus. Conservation Genetics, 11(3), 1115–1118. https://doi.org/10.1007/s10592-009-9893-1 Cite
Baminger, H., Locher, R., & Baur, B. (2000). Incidence of dart shooting, sperm delivery, and sperm storage in natural populations of the simultaneously hermaphroditic land snail Arianta arbustorum. Canadian Journal of Zoology, 78(10), 1767–1774. https://doi.org/10.1139/z00-113 Cite
Bisenberger, A., Baumgartner, G., Kleewein, D., & Sattmann, H. (1999). Untersuchungen zur Populationsökologie von Cylindrus obtusus (DRAPARNAUD, 1805) (Pulmonata, Helicidae). Annalen des Naturhistorischen Museums in Wien, Serie B, 101B, 453–464. Cite
Cadahía, L., Harl, J., Duda, M., Sattmann, H., Kruckenhauser, L., Fehér, Z., … Haring, E. (2014). New data on the phylogeny of Ariantinae (Pulmonata, Helicidae) and the systematic position of Cylindrus obtusus based on nuclear and mitochondrial DNA marker sequences. Journal of Zoological Systematics and Evolutionary Research, 52(2), 163–169. https://doi.org/10.1111/jzs.12044 Cite
Duda, M., Kruckenhauser, L., Haring, E., & Sattmann, H. (2010). Habitat requirements of the pulmonate land snails Trochulus oreinos oreinos and Cylindrus obtusus endemic to the Northern Calcareous Alps. Austria.- eco.mont / Journal on Protected Mountain Areas Research, 2, 5–12. https://doi.org/10.1553/eco.mont-2-2s5 Cite
Kruckenhauser, L., Haring, E., Tautscher, B., Cadahía, L., Zopp, L., Duda, M., … Sattmann, H. (2017). Indication for selfing in geographically separated populations and evidence for Pleistocene survival within the Alps: the case of Cylindrus obtusus (Pulmonata: Helicidae). BMC Evolutionary Biology, 17, 138. https://doi.org/10.1186/s12862-017-0977-0 Cite
Schileyko, A., Baminger, H., & Sattmann, H. (1997). On the variability of the distal genital tract of Cylindrus obtusus (DRAPARNAUD, 1805) (Gastropoda, Helicidae). Annalen des Naturhistorischen Museums in Wien, Serie B, 99B, 535–538. Cite
Zopp, L., Haring, E., Kruckenhauser, L., Schileyko, A., & Sattmann, H. (2017). Anatomical and morphological variation in the eastern Alpine endemic Cylindrus obtusus (Draparnaud, 1805) (Gastropoda: Helicidae). Journal of Molluscan Studies, 83(3), 316–324. https://doi.org/10.1093/mollus/eyx022 Cite

Abstracts

Cadahía, L., Harl, J., Sattmann, H., Duda, M., Luis, Kruckenhauser, L., Fehér, Z., … Haring, E. (2014). Phylogenetic relationships of Cylindrus obtusus (Pulmonata, Helicidae) deduced from nuclear and mitochondrial DNA marker sequences. Presented at the Workshop Alpine Land Snails 2014, Johnsbach, Austria.
Cadahía-Lorenzo, L., Harl, J., Sattmann, H., Duda, M., Kruckenhauser, L., Fehér, Z., … Haring, E. (2013). New insights on the phylogenetic position of the helicid species Cylindrus obtusus based on nuclear and mitochondrial DNA marker sequences. Presented at the World Congress of Malacology, Ponta Delgada, Azores (Portugal).
Cadahía-Lorenzo, L., Harl, J., Sattmann, H., Duda, M., Kruckenhauser, L., Fehér, Z., … Haring, E. (2013). The phylogenetic position of the enigmatic helicid species Cylindrus obtusus deduced from nuclear and mitochondrial DNA marker sequences. Presented at the NOBIS Young Researchers´ Day, Vienna, Austria.
Cadahía-Lorenzo, L., Harl, J., Sattmann, H., Duda, M., Kruckenhauser, L., & Haring, E. (2013). New insights on the phylogenetic position of the helicid species Cylindrus obtusus based on nuclear and mitochondrial DNA marker sequences. Presented at the BioSyst EU 2013, Vienna, Austria.
Haring, E., & Sattmann, H. (2011). Opinions on the origin of Cylindrus obtusus (Gastropoda, Helicidae). Talk presented at the Exkursionsworkshop Alpine Landschnecken, Johnsbach, Austria.
Kruckenhauser, L., Haring, E., Däubl, B., Cadahía, L., Zopp, L., & Sattmann, H. (2010). Selfing in geographically separated populations of Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae) – speciation in progress? Poster presented at the Conference “Speciation Research 2010″, Laxenburg (Austria).
Kruckenhauser, L., Sattmann, H., Däubl, B., Zopp, L., Haring, E., & Cadahía, L. (2011, June). Selfing in geographically separated populations of Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae). Poster presented at the 6th Congress of the European Malacological Societies, Vitoria-Gasteiz (Spain).
Kruckenhauser, L., Sattmann, H., Däubl, B., Cadahía, L., Zopp, L., & Haring, E. (2011). Selfing in geographically separated populations of Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae). Poster presented at the Exkursionsworkshop Alpine Landschnecken, Johnsbach, Austria.
Kruckenhauser, L., Sattmann, H., Däubl, B., Cadahía, L., Zopp, L., & Haring, E. (2010). Phylogeography of a high Alpine Austrian endemic: Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae). Oral presentation presented at the World Congress of Malacology, Phuket (Thailand).
Kruckenhauser, L., Haring, E., Däubl, B., Cadahía, L., Zopp, L., & Sattmann, H. (2010, December). Population genetics and phylogeography of Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae), an endemic of the Austrian Alps. Oral presentation presented at the 4. Jahrestagung NOBIS Austria, Salzburg (Austria).
Kruckenhauser, L., Zopp, L., Sattmann, H., Däubl, B., Cadahia, L., & Haring, E. (2013). Snails do it – but sometimes they don´t need to do it! Selfing in geographically separated populations of Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae). Presented at the World Congress of Malacology, Ponta Delgada, Azores (Portugal).
Kruckenhauser, L., Zopp, L., Sattmann, H., Däubl, B., Cadahía, L., & Haring, E. (2014). Snails do it – but sometimes they don´t need to do it! – Selfing in geographically separated populations of Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae). Presented at the Workshop Alpine Land Snails 2014, Johnsbach, Austria.
Kruckenhauser, L., Haring, E., Tautscher, B., Cadahía, L., Zopp, L., Duda, M., … Sattmann, H. (2017). Indication for selfing in geographically separated populations of the pulmonate land snail Cylindrus obtusus (Pulmonata: Helicidae). Presented at the 110. Jahrestagung der Deutschen Zoologischen Gesellschaft, Universität Bielefeld.
Kruckenhauser, L., Haring, E., Tautscher, B., Cadahía, L., Zopp, L., Duda, M., … Sattmann, H. (2017). Anatomical and biological peculiarities in Cylindrus obtusus (Pulmonata: Helicidae) an endemic land snail from the Eastern Alps. Presented at the ISEM7 October, 4-7, 2017, Sutomore Montenegro.
Zopp, L., Kruckenhauser, L., Haring, E., Harl, J., Schileyko, A., & Sattmann, H. (2011). Geographic differences in genital anatomy and histology among populations of Cylindrus obtusus (Draparnaud, 1805) (Gastropoda: Helicidae). Poster presented at the Exkursionsworkshop Alpine Landschnecken, Johnsbach, Austria.
Zopp, L., Kruckenhauser, L., Haring, E., Harl, J., Schileyko, A., & Sattmann, H. (2010). Do geographically distinct populations of Cylindrus obtusus (Gastropoda: Pulmonata: Helicidae) differ in the mode of reproduction? Poster presented at the The Malacological Society of London, Molluscan Forum, London (UK).
Zopp, L., Kruckenhauser, L., Haring, E., Sattmann, H., Harl, J., Schileyko, A., & Sattmann, H. (2011, June). Geographic differences in genital anatomy and histology among populations of Cylindrus obtusus (Draparnaud, 1805) (Gastropoda: Helicidae). Poster presented at the 6th Congress of the European Malacological Societies, Vitoria-Gasteiz (Spain).
Zopp, L., Kruckenhauser, L., Haring, E., Schileyko, A., & Sattmann, H. (2011, December). Does the hermaphroditic land snail Cylindrus obtusus (Draparnaud, 1805) always use the same mode of reproduction? (Gastropoda: Helicidae). Oral presentation presented at the 5. Jahrestagung NOBIS Austria, Salzburg (Austria).
Zopp, L., Kruckenhauser, L., Haring, E., Schileyko, A., & Sattmann, H. (2011, November). Geographic variation in the reproductive mode of Cylindrus obtusus (Draparnaud, 1805) (Gastropoda: Helicidae). Oral presentation presented at the The Malacological Society of London, Molluscan Forum, London (UK).

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